Miniaturization has the potential to improve sensitivity with the added benefit of portability, reliability, and sensitivity, which is ideal for trace analysis with small samples and biological samples. In developing the micro version, we printed the arrays on solid surfaces. Preliminary studies have been done where sensing elements were printed on various microscopic slides coated with epoxy-silane, amino-silane, lysine, polyacrylamide, nitrocellulose, silica gel, or other coatings. Through collaborations with KIT and Arrayjet, printing was performed using DPN® technology and non-contact microarray piezoelectric technology, respectively; as well as in-house with a modified inkjet printer. The original eight DETECHIP® sensors were printed at various concentrations and the morphology and array formats were evaluated. The assays were visualized in color with a flat-bed document scanner and microscope, while fluorescence was visualized using a microarray reader Axon 4000A.
Watch how Micro-DETECHIP works:
Watch how Micro-DETECHIP is analyzed: